- Epigenetics of AAV episome using nanpore and CUT&Tag sequencing
- Interrogating nucleotide patterns in the AAV genome sequence
- Engineering of AAV capsids through rational design
- Hybrid virus design using AAV and B19V
- Deciphering the impact of engineered ITR sequences on AAV vector production & function
- Alternative cargo modalities in AAV vectors
- Analysis of AAV NGS Data
- Development of methods and bioinformatics pipelines for NGS Data analysis
- Rational engineering and retargeting of AAV vectors for CNS gene therapy approaches
- Humanized experimental models to evaluate AAV tropism and translational relevance
- In vivo AAV library screens in major organs
- Genetic code expansion using AAV vectors
- AAV vector retargeting through combinatorial peptide display
- Analysis of AAV next-generation sequencing data (Illumina, PacBio)
- Establishment of user-friendly AAV alignment and analysis tools
- Directed evolution of AAV vectors in the liver, eye and kidney
- Experimental & bioinformatic high-throughput screening pipelines
- Development of novel gene therapy vectors derived from Bocarvirus
- Engineering of synthetic circuits to control bocavirus vector production
- Directed evolution of AAV vectors in the liver, eye and kidney
- Experimental & bioinformatic high-throughput screening pipelines
- Combinatorial CRISPR/RNAi strategies against HBV/HDV
- AAV engineering for expression of broady neutralizing antibodies
- AAV Rep protein engineering
- AAV screening in the CNS and musculature
- Elucidating the intracellular dynamics of Bocarvirus capsids as viral vectors
- Capsid engineering of bocavirus to improve transduction & identifying physicochemical properties of bocavirus
- all aspects of AAV production for internal and external use